Cell surface IL-12Rβ1 chain expression in HEKC293 cells transfected with wild-type and mutated IL-12Rβ1 genes.

An expression vector carrying the wild-type (A) or mutated (B) IL-12Rβ1 chain–encoding gene was transfected into HEKC293 cells. Transfected HEKC293 cells were selected with hygromycin, stained with a monoclonal antibody against IL-12Rβ1 chain (black) or a control IgG-1 monoclonal antibody 0.5β (white), stained with an FITC-labeled, goat-antimouse IgG, and subjected to flow cytometry.