American Society of Hematology

Fig. 8.

$Fig. 8. The distribution of TfRs between cell-surface and intracellular compartments is similar in C282Y and WT cells. / Cell-surface (external) and total cellular Tf binding sites were measured by 125I-Tf binding to C282Y and WT cells at 4°C and 37°C, respectively. Internal (intracellular) Tf binding sites represent the difference between 125I-Tf binding at 37°C and 4°C. The fraction of intracellular 125I-Tf binding sites was also determined by washing cells from the 37°C binding assay with an acidic buffer to remove cell-surface–bound125I-Tf, as discussed in the text. Bars represent means ± SE (n = 4).$

The distribution of TfRs between cell-surface and intracellular compartments is similar in C282Y and WT cells.

Cell-surface (external) and total cellular Tf binding sites were measured by ¹²⁵I-Tf binding to C282Y and WT cells at 4°C and 37°C, respectively. Internal (intracellular) Tf binding sites represent the difference between ¹²⁵I-Tf binding at 37°C and 4°C. The fraction of intracellular ¹²⁵I-Tf binding sites was also determined by washing cells from the 37°C binding assay with an acidic buffer to remove cell-surface–bound¹²⁵I-Tf, as discussed in the text. Bars represent means ± SE (n = 4).

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