Fig. 7.
![Fig. 7. Pulse-chase analysis of cells cotransfected with GPIIIa and either normal GPIIb, mutant [674R]GPIIb, or equimolar amounts of both GPIIb cDNAs. / CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C]GPIIb, mutant [674R]GPIIb, or equimolar amounts of both cDNAs, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with either anti-GPIIb or anti-GPIIIa. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2640/6/h80910985007.jpeg?Expires=1725012497&Signature=ze2LqVdQ2kpcm4E-na4c24P0X~LFFGNoEL5~oS1~QLWAnB3GUHDCFvRKR4YC0NjphkwC2eHOy83cLNlK~66Vw~L5oUl7w4YBpcxFAU6tUxSKtss3-SOMXoSlF3KHM4IEKvx0AOjlT6ysZY2fJTIK0ILlKyxobok3f~REILJALZBj4DzdscjuWXxBChLJ~AwYNCm2IyUpfww~-MSCKpKSrTJggvkk9of-HnqVuyM-3Emr~jJL2xBd8FeuTHR-RM0oxLzwzSrHhb7D6wAFtRJ0yPJs8b7JoFwcTJIyrKbzlp3h4gmAXh6TQefNuWh6pNxL0ybmk5bzbGgUwFy-PAniZw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of cells cotransfected with GPIIIa and either normal GPIIb, mutant [674R]GPIIb, or equimolar amounts of both GPIIb cDNAs.
CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C]GPIIb, mutant [674R]GPIIb, or equimolar amounts of both cDNAs, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with either anti-GPIIb or anti-GPIIIa. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”
