Fig. 4.
![Fig. 4. Effects of substitutions of 674C and 687C of GPIIb in the surface exposure and BiP association of GPIIb-IIIa complexes. / CHO-IIIa cells were transfected with the cDNA encoding normal [674C]GPIIb or mutant forms of GPIIb, as indicated. In the upper panel, the surface expression of GPIIb-IIIa complex was determined by flow cytometry, using an anti-GPIIb moAb. The results are expressed as a percentage of the surface exposure of normal [674C]GPIIb-IIIa complexes. The results are the means ± SEM of at least 3 independent experiments in duplicate. In the lower panel, immunoprecipitation of the cell lysates and Western blot analysis of the precipitates were performed as described in “Materials and methods.”](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2640/6/h80910985004.jpeg?Expires=1725012667&Signature=rzVLen1N67LaFW3O0znKODsCBuZXewh~ckyV9auVttdCvBbPPGm9MH5EnfD36Kfz6pqtKB8F33MqMvRDmwQDuJ3KERK8Go~UDMfadwHQuYAuBGemnPUviWP3457iz1nolK-6YQNw2puAa2nusPDYkybgwK1KQ5wewzymWDi0R~ii4P~NU~e2VtyPnnwWx71IPLDtNGBhu~Q4Sl3YNfoa2JU8fhNT~Nid6SFlCHP6-ZCotvTuSzO~dTBurMH2daWGstA4VTLQ6Ev6~sf2eZG381vMQ9WPDh5CZeAJDVsjsTHlBLdE9EkMOz0Xuo32fNKKuq97g1c~62yAx0TVsuaJcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of substitutions of 674C and 687C of GPIIb in the surface exposure and BiP association of GPIIb-IIIa complexes.
CHO-IIIa cells were transfected with the cDNA encoding normal [674C]GPIIb or mutant forms of GPIIb, as indicated. In the upper panel, the surface expression of GPIIb-IIIa complex was determined by flow cytometry, using an anti-GPIIb moAb. The results are expressed as a percentage of the surface exposure of normal [674C]GPIIb-IIIa complexes. The results are the means ± SEM of at least 3 independent experiments in duplicate. In the lower panel, immunoprecipitation of the cell lysates and Western blot analysis of the precipitates were performed as described in “Materials and methods.”
