Fig. 1.
![Fig. 1. Pulse-chase analysis of [674C] and [674R]GPIIb-IIIa synthesis. / CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C] or mutant [674R]GPIIb, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with anti-GPIIb. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2640/6/h80910985001.jpeg?Expires=1723201873&Signature=Lv0ehXEGVfXzejkFFZQ0V4MNvj7kUNM5OLcvgduS2BCf7PKSvcxCDfiJsbvYfUFjNVLB8-QHX2rr~VKkOhaPiPXWP5oR0rn0nKM7DTK6tEq4DjeuselXw8fqK66etMwSa92xL~4MrJAp70JsrEaVTl1CKU55pGnPUMHdsediH6F8-Vgu8DMDi1xBSmUBKx6lSha5D7u5r4MwFV30bqtost~IBDbMgKOJ6MhNHupJdazzGwH3KTz1NNnhkbrF2LZHcBTLfgBhu6EeI4TlAt9YZHkjE3gXNO4zke28w1TyVPN2ipRcjKXKsOFHPzpha-i0pAK16QmLuXE2r3bAqjd-Vg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of [674C] and [674R]GPIIb-IIIa synthesis.
CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C] or mutant [674R]GPIIb, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with anti-GPIIb. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”
