Fig. 4.
Effect of PD98059 on the phosphorylation of cytosolic PLA2.
Purified neutrophils were preincubated with PD98059 or DMSO vehicle at the doses indicated in the figure for 30 minutes before stimulation with 10 ng/mL GM-CSF for 5 minutes. Migration of cPLA2immunoprecipitates was determined by SDS-PAGE followed by Western blotting with an anti-cPLA2 antibody.
