Decreased expression of procaspase-2L does not inhibit the formation of the DISC.

Jurkat/neo and Jurkat/AS1 cells were treated for indicated times with recombinant FasL. Immunoprecipitation (IP) with an anti-Fas receptor Ab was followed by immunoblot detection of FADD, procaspase-8, and procaspase-2L. Controls include the Ab alone (mAb), beads incubated in the absence of Ab (beads), and Jurkat cell extracts (extract).