Western blot analysis of CD36-associated proteins.

Three detergents, CHAPS (lanes 1,2), Triton X-100 (lanes 3 and 4), and Brij 99 (lanes 5 and 6), were used to lyse the platelets. The immunoprecipitations with the anti-CD36 antibody (lanes 1, 3, and 5) were compared with those with nonimmune mouse IgG (lanes 2,4,6). The results show that αIIb, β3, α6, β1, and CD9 were detected in CHAPS and Brij 99 platelet lysates but were not detected in Triton X-100 platelet lysates. CD36 and the Src gene family members, Lyn, Fyn, and Yes, were detected in all of the detergent lysates.