EMSA analysis of NF-κB activation in IκB-M–expressing MEFs.

IκB-M–expressing normal and FANCC^−/− MEFs were treated with IFN-γ (10 ng/mL) and dsRNA (100 μg/mL), and EMSA was performed by using 5 μg of nuclear extracts and a kB oligonucleotide sequence as described in “Materials and methods.” The final 2 lanes indicate that binding reactions were incubated with 3 μg of anti-p65 (lane 7) or 3 μL of anti-p50 (lane 8) antibody.