American Society of Hematology

Fig. 2.

$Fig. 2. The surface phenotype of thymic DCs. / (A) By means of 4-color analysis, DCs were gated as HLA-DRhigh(Cy5)CD11b− (Texas Red) and HLA-DRhigh/intCD11b+. (B) Both fractions were then analyzed by means of FITC and PE channels. Particular cases: CD86 and IL-3Rα expressions were revealed in Texas-Red after the gating was carried out with FITC-conjugated anti-CD11b antibody. This analysis is representative of 6 separate thymus samples.$

The surface phenotype of thymic DCs.

(A) By means of 4-color analysis, DCs were gated as HLA-DR^high(Cy5)CD11b⁻ (Texas Red) and HLA-DR^high/intCD11b⁺. (B) Both fractions were then analyzed by means of FITC and PE channels. Particular cases: CD86 and IL-3Rα expressions were revealed in Texas-Red after the gating was carried out with FITC-conjugated anti-CD11b antibody. This analysis is representative of 6 separate thymus samples.

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