Fig. 7.
Influence of EDTA on platelet agglutination and activation by echicetin-IgMκ complex.
(A) Washed human platelets (500 μL, 5 × 108/mL) were stirred at 1100 rpm at 37°C in the presence (upper line) or absence (bottom line) of 5 mM EDTA and agglutinated by echicetin (5 μg) plus IgMκ (1 μg). (B) After 2 minutes of agglutination with echicetin-IgMκ complex, platelets were lysed by SDS. Proteins were separated by SDS-PAGE, transferred to a PVDF membrane, and stained with antiphosphotyrosine antibody (4G10).
