Fig. 6.
Agglutination and protein tyrosine phosphorylation induced by echicetin-IgMκ complex in washed human platelets.
(A) Washed human platelets (500 μL, 5 × 108/mL) were stirred at 1100 rpm at 37°C in the presence of 5 μg echicetin. One minute after adding echicetin, 1 μg IgMκ (upper curve) or buffer for control (bottom curve) were added. (B) Proteins from SDS-lysed platelets were separated by SDS-PAGE, transferred to PVDF membrane, and stained with antiphosphotyrosine antibody (4G10). The left panel shows proteins from echicetin-treated platelets; the right panel shows tyrosine phosphorylation of proteins from platelets activated by echicetin-IgMκ complex.
