Cell-cycle analysis of CD34⁺CD90⁺ and CD34⁺CD90⁻ progenitors from BMT donor (donor 4) at time of bone marrow harvest, and fully chimeric matched recipient (recipient D) 6 months after BMT.

(A) Flow cytometric analysis of donor CD34⁺CD90⁺ cells, after staining with Hoechst 33342 and pyronin Y. Analysis of the cellular uptake of these vital dyes allows the discrimination of G₀ (2N DNA + low levels of RNA), G₁ (2N DNA + higher levels of RNA), and S/G₂/M (greater than 2N DNA + higher levels of RNA) phases of the cell cycle. (B) Donor CD34⁺CD90⁻ cells. (C) Analysis of recipient's CD34⁺CD90⁺ cells 6 months after BMT. (D) Recipient CD34⁺CD90⁻ cells.