Sequences in the SMMHC portion of CBFβ-SMMHC inhibit CBFβ function.

(A) Schematic diagram of human CBFβ(p22), the CBFβ-SMMHC proteins generated by the inv(16), and experimentally truncated derivatives of human CBFβ and CBFβ-SMMHC. (B) Western blot documenting expression of the proteins illustrated in panel A inCbfb^−/−ES cells. (C) Colony formation byCbfb^−/−ES cells expressing the transgenes shown in panels A and B. Assays were performed with EBs following 10 days of primary differentiation. The mean values from 2 or 3 independent plates are shown from one experiment, representative of 3 independent experiments.