Fig. 1.
Correlation of flow cytometry and Southern blot analysis to detect human engraftment in NOD/SCID mice.
Data are from 2 representative animals transplanted with 0.6 × 106 human CD34+ PBSCs and killed 8 weeks after transplantation. (A) Southern blot analysis of the BM and spleen of 2 mice. Human:mouse DNA controls are given as percent human DNA. (B) BM and spleen isotype controls for FITC (x-axis) and PE (Y-axis). (C) Evaluation of human cells labeled with antihuman CD19-PE and CD45-FITC monoclonal antibodies in the BM and spleen. The percentage of human cells is indicated.
