K562/cE cells show a greater response to low-level band 3 coexpression than K562/D cells.

These histograms illustrate the data listed in Table 4 for K562/D+B3EQ clone 3 (left column) and in Table 5 for K562/cE+B3EQ clone 4 (right column). K562/D+pBn clone 2 and K562/cE+pBn clone 2 were used as negative controls. When low levels (ie, FL1 for BRIC 71 approximately equals 30, or 7 to 8 times higher than for K562/Rh+pBn clones) of B3 or B3EQ were coexpressed in K562/D cells, only a negligible increase in the binding of Rh polypeptide antibody and an approximately 1.5- to 2-fold increase in anti-RhAG binding were observed. However, when the same amount of band 3 was coexpressed in K562/cE cells, BRIC 69 binding was increased 2- to 3-fold, and LA18.18 binding was increased 3- to 5-fold.