Production of IFN-γ by thymocytes and splenocytes from control and ZAP-70^−/− mice.

Sorted NK1.1⁺ TCRαβ⁻ splenocytes from control mice (open bar), NK1.1⁺TCRαβ⁻ splenocytes (shaded bar), and NK1.1⁺ TCRαβ⁻ thymocytes (closed bar) from ZAP-70^−/− mice were cultured in medium alone (medium) or with immobilized anti-NK1.1 mAb (NKR-P1) in the presence of rhIL-2 (1000 U/mL). Then, IFN-γ in the culture supernatants were quantified by enzyme-linked immunosorbent assay. Results are representative results of 3 separate experiments.