Phenotype of NK1.1⁺CD3⁻thymocytes from ZAP-70^−/−, RAG-1^−/−, and B6.

Whole thymocytes (B6-RAG-1^−/−), thymocytes enriched for CD8⁻ HSA⁻ population with MACS (ZAP-70^−/−), and thymocytes enriched for CD8⁻ HSA⁻ with MACS and electronic gating for CD3⁻ thymocytes (B6 mice) were stained with PE–anti-NK1.1 and various cell surface markers (grouped in panels A-C) as described in “Materials and methods.” Expressions of NK1.1 (vertical) and other markers (horizontal) on either enriched or whole thymocytes are indicated.