DX5 expressions on NK1.1⁺ thymocytes and splenocytes from control (ZAP-70^+/−) and ZAP-70^−/− mice.

Thymocytes and splenocytes were stained with PE–anti-NK1.1, FITC–anti-TCRαβ, and biotinylated anti-DX5 followed by streptavidin–Red 670. NK1.1⁺ TCRαβ⁺ or NK1.1⁺ TCRαβ⁻ cells in the thymus and spleen were electronically gated and analyzed for the expression of DX5 on FACScan. Dead cells were electronically gated out with propidium iodide staining. FACS profiles were shown in contour with NK1.1 versus DX5 staining.