Synergistic effect of Regakine-1 and IL-8 on neutrophil chemotaxis.

Different concentrations of IL-8 (0-10 ng/mL) and pure natural Regakine-1 (0-300 ng/mL) were combined in the lower compartment of the microchamber to measure neutrophil chemotaxis (A). Alternatively (B), human neutrophils were incubated with different concentrations (0-100 ng/mL) of natural Regakine-1 (10 minutes, 37°C) before transfer to the upper compartment of the microchamber to measure the chemotactic response to various concentrations of IL-8 (0-100 ng/mL). The mean CIs are derived from 3 independent experiments. The SEM did not exceed 20% of the mean CI and is omitted for clarity.