Presence of retroviral vector marker sequences in bone marrow of mice 6 months after in utero transplantation of transduced FLCs.

Standard dilutions of transduced and nontransduced MPS VII cells were assayed as a standard. (A) φX174 HaeIII marker. (B) 100% transduced cells. (C) 1 in 10 dilution (D) 1 in 10² dilution. (E) 1 in 10³ dilution. (F) 1 in 10⁴ dilution. (G) Pure nontransduced cells. (H) H₂O control. The PCR bands amplified from the bone marrow of 6-month-old mice that had been transplanted in utero (panels J-M) were compared with the standards. The PCR bands of panels J-L were between reference bands E and F and thus were scored as the lower value of 10⁴, but no band was detected in panel M, which was scored negative.