American Society of Hematology

Fig. 4.

$Fig. 4. Engraftment of human cells in NOD/SCID mice: A10-GFP virus GFP. / A10–GFP- or GFP-transduced progenitors and the nontransduced cell fraction were injected into NOD/SCID mice directly after finishing transduction. Engraftment of human lymphoid and myeloid cells was assessed by quantifying CD19+/CD34− or CD15+12 weeks after transplantation by FACS analysis. The FACS profile of the BM of a representative mouse transplanted withHOXA10-transduced and nontransduced fetal liver cells is shown at 12 weeks after transplantation. Percentages of GFP+ and GFP− human lymphoid and myeloid cells are presented as proportions of the human GFP+ and GFP− CD45+ cell populations, respectively (A). Ratios of human lymphoid and myeloid cells were calculated for the individual mice in the GFP and A10-GFP experimental groups (n = 7 and n = 11, respectively) for both the GFP+ and the GFP− compartments. Median ratios are indicated (B).$

Engraftment of human cells in NOD/SCID mice: A10-GFP virus GFP.

A10–GFP- or GFP-transduced progenitors and the nontransduced cell fraction were injected into NOD/SCID mice directly after finishing transduction. Engraftment of human lymphoid and myeloid cells was assessed by quantifying CD19⁺/CD34⁻ or CD15⁺12 weeks after transplantation by FACS analysis. The FACS profile of the BM of a representative mouse transplanted withHOXA10-transduced and nontransduced fetal liver cells is shown at 12 weeks after transplantation. Percentages of GFP⁺ and GFP⁻ human lymphoid and myeloid cells are presented as proportions of the human GFP⁺ and GFP⁻ CD45⁺ cell populations, respectively (A). Ratios of human lymphoid and myeloid cells were calculated for the individual mice in the GFP and A10-GFP experimental groups (n = 7 and n = 11, respectively) for both the GFP⁺ and the GFP⁻ compartments. Median ratios are indicated (B).

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