Presence of p210^BCR/ABL in normal CD34⁺ cells prevents adhesion-mediated proliferation inhibition.

Cord blood CD34⁺ cells (5 × 10⁶) were transduced on 3 consecutive days with the control vector, eGFP, or with p210-eGFP and were plated in fibronectin or poly-L-lysine–coated dishes for 12 hours in the presence of the β1-integrin activating antibody, 8A2. Nonadherent and adherent cells were collected separately, cells were fixed and incubated with propidium iodide, and the cell cycle status of CD34⁺/eGFP⁺ cells was analyzed by FACS using Modfit-LT software. A representative example of fibronectin-nonadherent and fibronectin-adherent p210-eGFP–transduced cells is shown.