Comparison of the effects of STI571 on cellular tyrosine phosphorylation in Ba/F3 cells expressing BCR/ABL, TEL/ABL, TEL/ARG, TEL/PDGFR, or TEL/JAK2.

(A) An anti-pTYR immunoblot showing cellular tyrosine phosphorylation in untransfected Ba/F3 cells and TEL/ARG-Ba/F3 cells cultured in the presence and absence of STI571 (left panel). An anti-pTYR immunoblot showing cellular tyrosine phosphorylation in untransfected Ba/F3 cells, TEL/ABL-Ba/F3, and Ba/F3.p210 cells cultured in the presence and absence of STI571 (right panel). Immunoblots were stripped and incubated with anti–β-actin as a loading control. (B) Effect of 24-hour STI571 treatment on viability of cells analyzed in part A. (C) Anti-pTYR immunoblots showing cellular tyrosine phosphorylation in TEL/ABL-Ba/F3, TEL/ARG-Ba/F3, Ba/F3.p210, TEL/PDGFR-Ba/F3, TEL/JAK2-Ba/F3, and untransfected Ba/F3 cells treated with increasing concentrations of STI571. Immunoblots were stripped and incubated with anti–β-actin as a loading control.