Defective cyclin B1- and total protein-ubiquitination in lymphocytes from HIV-infected patients.

Protein and cyclin B ubiquitination were studied after in vitro activation (ConA + IL-2) of peripheral blood lymphocytes from 20 HIV-infected patients and 20 controls, and measured at different time points (0, 40, 70, and 90 hours). (A) Level of total protein ubiquitination. (B) Level of cyclin B1 ubiquitination. Both curves show results of a representative experiment in which sequential cyclin B and protein ubiquitination in lymphocytes from one HIV-infected patient and one control were analyzed in the same Western blot experiment (3 measurements per patient per time point). The bottom part of the figure shows pictures of 2 representative gels, one for total protein ubiquitination and one for cyclin B ubiquitination, as sequentially determined at 40 and 90 hours after activation in one HIV-infected patient and one control.