Impaired cyclin B1 degradation in lymphocytes from HIV-infected patients.

Kinetic analysis of degradation of the newly synthesized cyclin B1 in peripheral blood lymphocytes from HIV-infected patients and controls. In these experiments cells were pulse labeled for 4 hours with³⁵S-methionine after 24 hours in vitro activation with ConA. Cyclic B degradation was calculated as a percentage of radioactivity associated with the protein band after a 20-, 40-, and 90-minute chase period. The curves in the top panel and the numeric results in the bottom panel represent the mean of 20 patients and 20 uninfected controls.