Intracellular turnover of cyclin B1 in lymphocytes from HIV-infected patients.

Kinetic analysis of intracellular concentration of cyclin B1 (expressed as percentage of the baseline pixel intensity) on in vitro mitogen activation was performed in peripheral blood lymphocytes from 20 HIV-infected patients and 20 controls. The top panel reports Western blots from selected experiments in which the cyclin B1 overtime cellular expression was sequentially studied after lymphocyte activation. The curve in the bottom panel describes results of a representative experiment in which sequential cyclin B expression in one HIV-infected patient and one control were analyzed in the same Western blot experiment (3 measurements per patient per time point). The numeric results in the box represent the mean levels of initial cyclin B synthesis as studied at time 0, 24, and 72 hours after activation in the same representative experiment.