Double-immunofluorescence staining for TF and VIIa.

BHK(TF) cells were incubated with 10 nM VIIa for 1 hour at 37°C and then stained for VIIa (A) and TF (B) as described in “Materials and methods.” (C) Pictures of panels A and B have been merged to highlight areas with colocalization (yellow). Note that most of the cells show a high degree of colocalization of TF and VIIa. In a subset of these, colocalization in perinuclear vesicles is quite evident.