EKLF-ER™ induced activation of the human β-globin gene.

RNAse protection assays of total RNA from A2 (A) and B1 (B) cell lines treated for 72 hours in ethanol (minus sign) or 4-hydroxytamoxifen (plus sign). The human β-globin gene was induced in most cell lines in response to 4-hydroxytamoxifen, whereas the level of human γ-globin expression remained elevated at levels similar to that of the endogenous mouse α-globin gene. The parent cell lines A2 and B1 did not express any β-globin in response to 4-hydroxytamoxifen treatment.