Inhibition of endothelial cell adhesion receptor expression by adhesion-blocking mAbs against P-selectin, ICAM-1, and VCAM-1.

(A) Effect of mAbs directed against P-selectin (G1, 10 μg/mL) and ICAM-1 (HU 5/3, 10 μg/mL) on VCAM-1 and ICAM-1 expression after 6 hours of endothelial cell coculture with M2 AML blasts (10⁷blasts/25-cm² flasks). Endothelial cells were analyzed for VCAM-1 and ICAM-1 expression by flow cytometry. (B) Effect of mAbs directed against and VCAM-1 (HAE-3, 10 μg/mL) or ICAM-1 (HU 5/3, 10 μg/mL) on E-selectin and ICAM-1 expression after 6 hours of endothelial cell coculture with M5 AML myeloblasts (10⁷blasts/25-cm² flasks). Endothelial cells were analyzed for E-selectin and ICAM-1 expression by flow cytometry. Dotted lines indicate background staining of endothelium labeled with an unreactive isotype-matched mAb. Dashed lines indicate expression of ICAM-1 by unactivated endothelium.