Fig. 3.
Kinetics of VCAM-1 and E-selectin expression by endothelial cells cocultured in 25-cm2 flasks containing 107 M5 AML blast cells.
Endothelial cells, detached with 5 mM EDTA at the indicated times, were analyzed for VCAM-1 and E-selectin expression by flow cytometry. Results are representative of 3 similar experiments. Background staining obtained with an unreactive isotype-matched mAb is indicated by dotted lines in the left panels.