A representative Western blot analysis showing DNA-PKcs and BCR-ABL expressions in doxycycline-inducible Ba/F3 cell line.

Cells were grown in IL-3 (10% WEHI), with (+, lane 1) and without (−, lane 2) doxycycline (1 μg/mL) for 48 hours. Cells extracts were immunoblotted with anti-DNA-PKcs and anti-BCR-ABL antibodies. Equal protein loading was assessed by protein dosage, Ponceau staining, and the presence of an equal intensity of the endogenous c-abl. When the cells were deprived of tetracycline for 48 hours, a marked increase of BCR-ABL expression was found, along with a reduction of DNA-PKcs expression.