Fig. 9.
Tyrosine phosphorylation of Stat-5 protein in M1/βc cells with various α subunits.
(A) Immunoprecipitation–immunoblotting analysis. Cells were treated with rhGM-CSF 100 ng/mL for 10 minutes. Lysates were then immunoprecipitated with anti–Stat-5. Precipitated proteins were then solubilized, divided, and subjected to immunoblotting with either antiphosphotyrosine (top panel) or anti–Stat-5 (bottom panel). (B) Quantitation by densitometry of tyrosine-phosphorylated Stat-5 band after rhGM-CSF treatment.
