Identical phenotype of enriched dendritic cells from normal and IL-7Rα^−/− males.

Dendritic cells were enriched from male splenocytes following plate adherence and incubation with IL-4 and GM-CSF as described in “Materials and methods.” Before injection, portions of the cells were analyzed by flow cytometry. Staining with FITC-conjugated antibodies expressed by dendritic cells were analyzed against a cocktail of lineage-specific antibodies conjugated to phycoerythrin (PE). The dashed circle in each graph delineates the dendritic cells. Staining for PE in the indicated cells represents intermediate labeling with anti-CD11b with no expression of B220 or CD3 (data not shown), a finding consistent with the observations of other investigators.65 The phenotype of dendritic cells from normal (top panels) and IL-7Rα^−/−mice (bottom panels), as characterized by this set of markers, was identical. MHC indicates major histocompatibility complex.