Expression of CD58, ninjurin1, creatine kinase B, and Ref1 in normal and leukemic immature B cells by flow cytometry.

For each protein, results obtained with 2 normal bone marrow samples and 2 cases of B-lineage ALL are shown. Flow cytometric dot plots illustrate labeling of CD19⁺ lymphoid cells with antibodies against the tested protein (FITC; x axes) and with anti-CD10 (PE; y axes). Drawing of quadrants was based on staining with isotype-matched fluorochrome–conjugated nonreactive Igs. The percentage of cells in the upper right quadrant is indicated in each plot.