Northern blot analysis of inflammatory markers.

HUVECs were transduced with AdV-dnIKK2 or control virus (AdV-GFP) or left without virus and after 2 days stimulated with IL-1 or TNFα for 4 hours as indicated. VCAM-1–, IL-8–, and E-selectin–specific bands are indicated. Equivalent loading and transfer of RNA was confirmed by hybridization with GAPDH.