Activation of NF-κB is inhibited in dnIKK2-expressing cells.

(A) Nuclear translocation of p65–NF-κB. Control HUVECs (i-ii) or HUVECs that had been transduced with control virus (iii-iv) or AdV-dnIKK2 (v-vi) were stimulated with TNFα for 1 hour (ii,iv,vi) or left unstimulated (i,iii,v). Nuclear translocation of NF-κB was revealed by immunofluorescence, using an anti-p65 antibody. (B) Nuclear extracts were prepared from control cells, AdV-GFP, and from AdV-dnIKK2–transduced HUVECs and were analyzed by electrophoretic mobility shift assay. The NF-κB–specific bands are indicated by arrows.