Fig. 3.
Fig. 3. Gene expression for Gr B and perforin in anti–H-2d CTLs derived from +/+,. / mi/mi, and tg/tg mice.Total RNA (3.0 μg) was extracted from the aliquots of CTLs, blotted, and hybridized with the Gr B or perforin probe. Reprobing with β-actin probe verified an RNA equal loading.

Gene expression for Gr B and perforin in anti–H-2d CTLs derived from +/+,

mi/mi, and tg/tg mice.Total RNA (3.0 μg) was extracted from the aliquots of CTLs, blotted, and hybridized with the Gr B or perforin probe. Reprobing with β-actin probe verified an RNA equal loading.

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