Endothelial activation in murine tissues.

Tissues were scored (per “Materials and methods”) for endothelial expression of E-selectin (left), ICAM-1 (middle), and VCAM-1 (right). Large vessels (top row) and capillaries (bottom row) were scored separately. Tissues examined were heart (H), kidney (K), liver (L), and spleen (S). Data are shown as the mean ± SD (n = 4-8 different mice for each bar). Data from untreated mice with sickle cell disease are shown by stippled bars; data from sulfasalazine-treated mice with sickle cell disease are shown by filled bars. The asterisk indicates differences that are statistically significant. The apparent absence of a bar for E-selectin in the large vessels of the heart of treated animals (upper left panel) is because that value was 0 ± 0.