Migration assays in vitro.

Chemokine-induced in vitro migration of leukocytes from lymph nodes with HD. Shown are the mean numbers (SE) of migrated cells per 5 high-power fields (triplicate wells) (5HPF) in the presence of increasing molar (M) concentrations of IP-10, RANTES, and MIP-1β. PHA-activated peripheral blood lymphocytes were used as a positive control to confirm the functionality of the migration assay.