s-FKN activates Syk and Src tyrosine kinases in MonoMac6 cells.

MonoMac6 cells were incubated with 60 nM s-FKN for the indicated times. Src (A) and Syk (B) kinases were immunoprecipitated (IP) with specific antibodies from cell lysates, resolved by SDS-PAGE, and electrophoretically transferred to Immobilon-P membrane. Src activity was assessed by in vitro kinase assay using enolase as substrate, and Syk activity by immunoblotting using antiphosphotyrosine 4G10 monoclonal antibodies. The amounts of Syk and c-Src immunoprecipitated were evaluated by Western blotting (WB) analysis with the appropriate antibodies. The results are representative of 2 experiments.