Fig. 5.
The PKC activators BRY, PMA, and MEZ, but not PLC, promote apoptosis in LC-treated cells.
U937 cells (2 × 105 cells/mL) were exposed to lactacystin (LC; 1 μM) and either bryostatin (BRY; 10 nM), phorbol myristate acetate (PMA; 10 nM), mezerein (MEZ; 10 nM), phospholipase C (PLC; 200 mU/mL), or an inactive phorbol control (4α-PMA; 10 nM) for 24 hours. Values represent the means for triplicate experiments (± SEM) and are expressed as the percentage of apoptotic cells determined by morphologic analysis.
