Examples of CGH and FISH results.

CGH-karyotype (A) and ratio profile (B) obtained in case 19. The vertical green line is the threshold for overrepresentations; the vertical left red line is the threshold for underrepresentation. Gains (green) and losses (red) are blotted next to the ideogram. Heterochromatic regions (on chromosomes 1, 9, 13, 14, 15, 21) and chromosomes 19, 22, and Y were excluded from analysis. (C) FISH with centromeric probes for chromosome 6 (green) and 3 (red) performed on a frozen section from case 6 that showed overrepresentation of chromosome 3. 3 green and 6 red signals found in the “popcorn” cell confirmed the CGH result and indicated the triploid status of the analyzed cell. (D) Interphase-FISH with the chromosome 1 centromeric probe (red) and the chromosome 6 centromeric probe (green) in case 9. Malignant cells were hallmarked by double red signal due to the idic(1)(q10) (panel E). Note presence of 2 or 3 green signals in malignant cells. (E) Metaphase-FISH with the B5-2 cosmid (green) for BCL6 and the chromosome 1 centromeric probe (red) performed in case 9. Arrows indicate the split cosB5-2 signal on both derivatives of t(3;22)(q27;q11). (F) Partial metaphase-FISH with the cosB5-2 probe (green) in case 14 showing the split signal on der(3) (arrows) and on normal chromosome 3.