Inhibitory effects of methiothepin (a 5-HT₁/5-HT₂ receptor antagonist) on 5-HT–induced mRNA expression.

(A) TF mRNA expression. (B) PAI-1 mRNA expression. Quiescent RAECs were preincubated with the indicated concentrations of methiothepin for 30 minutes and then cultured with serotonin 1 μM for 2 or 3 hours. Relative TF and PAI-1 mRNA expression represent the fold increase in normalized TF and PAI-1 mRNA expression compared to vehicle-treated controls, respectively. Each bar represents the mean ± SEM of 3 independent experiments (*P < .05 versus RAECs treated with 5-HT only).