Apoptosis of AML M4 blasts in NOD SCID mice after adoptive transfer of activated CD4⁺Vα24NKT cells.

PBMCs from Pt 1 (AML M4) were intraperitoneally injected into 6 mice at 5 × 10⁵ cells/mouse. On day 5 after PBMC injection, 5 × 10⁴ CD4⁺Vα24NKT cells/mouse were intraperitoneally inoculated into 3 of the 6 mice. Mice were killed 24 hours after inoculation of the CD4⁺Vα24NKT cells, and peritoneal cells were harvested from each mouse. Apoptosis of AML PBMCs from Pt 1 in the peritoneal cells was assessed as described in Figure3. Apoptosis of PBMCs when only the PBMCs were injected into NOD SCID mice (A). Apoptosis of the PBMCs when activated CD4⁺Vα24NKT cells (B) or resting CD4⁺Vα24NKT cells (C) were transferred. The data are representative of 3 experiments with similar results.