Fine mapping of CTL epitopes recognized by clone 96.5.

The CTL clone 96.5 was derived from eGFP-stimulated PBMCs from animal Mm 96-97. (A) Initial mapping of eGFP epitopes. Eleven to 12–amino acid peptides, overlapping by 8 to 9– amino acids, were synthesized. Specific sequences were: 1A, MVSKGEELFTGV; 1B, GEELFTGVVPIL; 1C, FTGVVPILVELD; and 1D, VVPILVELDGD. Autologous B-LCLs were sensitized with 100 μg/mL and tested for lysis using clone 96.5 at an effector-to-target ratio of 10:1. (B) Fine mapping of epitope specificity of CTL clone 96.5. Nine–amino acid peptides corresponding to the sequence of peptide 1A were used to sensitize target cells at the indicated concentrations. Peptide sequences were as follows: 1E (▴), FTGVVPILV; 1F (●), TGVVPILVE; 1G (▪), GVVPILVEL.