Detection of eGFP-specific CTLs in macaques following infusion of autologous CD34⁺ hematopoietic cells transduced with the LZRS/eGFP vector.

PBMCs were stimulated with autologous B-LCLs either expressing eGFP (A-C) or sensitized with eGFP peptides (D-G). PBMCs shown in G were obtained from an untransplanted control. Stimulated effector cells were examined for eGFP-specific CTL activity using transduced B-LCLs expressing eGFP or sensitized with eGFP peptides. The level of eGFP-expressing leukocytes at the indicated time after transplantation (PTx) is shown. Controls consisted of untransduced autologous B-LCLs.