Quantitation of eGFP expression in lymphocytes and granulocytes in macaques following reinfusion of transduced CD34⁺ cells.

Flow cytometric detection of eGFP in CD3⁺ T cells, CD20⁺ B cells, and granulocytes at sequential time points after transplantation with autologous CD34⁺ bone marrow cells transduced with LZRS/eGFP. Reported levels of eGFP expression reflect values obtained after subtraction of the background staining of concurrent negative controls consisting of blood samples from normal animals. Percent eGFP expression in negative controls was always less than 0.1%.