American Society of Hematology

Fig. 4.

$Fig. 4. Presence of primitive hematopoietic cells among CD41+ cells. / (A) LTC-IC assay. Proportion of negative wells in the LTC-IC assays at week 6. The frequency of LTC-ICs from cultured cord blood and adult mobilized CD34+ cells was calculated in the CD34+CD41−, CD34+CD41+CD42−, and CD34−CD41+CD42− cell fractions. (B) Phenotypic analysis of the human cells present in NOD-SCID cells 6 weeks after reconstitution with CD34+CD41− and CD34+CD41+ cells derived from cultured cord blood CD34+ cells. A triple staining was performed between a FITC anti-CD45 mAb, an R-PE-Cy5 anti-CD34 mAb, and R-PE anti-CD19, or CD11b or CD41 mAb. All these antibodies were human specific. The phenotype of human cells could be determined by gating cells on both their scatter properties and CD45 expression.$

Presence of primitive hematopoietic cells among CD41⁺ cells.

(A) LTC-IC assay. Proportion of negative wells in the LTC-IC assays at week 6. The frequency of LTC-ICs from cultured cord blood and adult mobilized CD34⁺ cells was calculated in the CD34⁺CD41⁻, CD34⁺CD41⁺CD42⁻, and CD34⁻CD41⁺CD42⁻ cell fractions. (B) Phenotypic analysis of the human cells present in NOD-SCID cells 6 weeks after reconstitution with CD34⁺CD41⁻ and CD34⁺CD41⁺ cells derived from cultured cord blood CD34⁺ cells. A triple staining was performed between a FITC anti-CD45 mAb, an R-PE-Cy5 anti-CD34 mAb, and R-PE anti-CD19, or CD11b or CD41 mAb. All these antibodies were human specific. The phenotype of human cells could be determined by gating cells on both their scatter properties and CD45 expression.

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