CD9 mAb treatment reduces the production of MK cells from CD34⁺ cells in liquid culture.

Purified CD34⁺ cells were cultured for 16 days under megakaryocytic liquid culture conditions in the presence or absence of SYB-1 CD9 mAb or of an isotype control mAb. One experiment of 4 is shown. Percentage (A) and total number (B) of CD41⁺ cells determined at several time-points of the culture. (C) Level of CD41 expression at various time-points of the culture, expressed as mean fluorescence intensity (MFI). (D) Variation of expression (MFI) of surface megakaryocytic markers in the presence of CD9 mAb compared to IgG1-treated cells on day 14 of the culture. NT, not treated.